In the interests of openness, we are pleased to share with you the Laboratory Standard Operating Procedures (LSOPs) for The University of Queensland Node. Please feel free to repurpose these to meet your own needs.
- LSOP01 - Adventitious Rooting Protocol
- LSOP02 - Arabidopsis Growing Procedure
- LSOP03 - Arabidopsis growth on Agar + seed sterilisation
- LSOP04 - Arabidopsis Protoplast Isolation
- LSOP05 - Arabidopsis Seed Surface Sterilisation
- LSOP06 - Phytohormone Extraction
- LSOP08 - DNA extraction
- LSOP09 - Enzymatic Sugar Measurements
- LSOP11 - Exudate Collection from hydroponic Arabidopsis Roots
- LSOP12 - Fluorescamine Method to Calculate Total Amino Acids
- LSOP13 - Gene Expression Analysis by Real Time
- LSOP15 - How to make GR24 treatment solutions for direct pea bud applications
- LSOP16 - Hydroponic Arabidopsis
- LSOP17 - Hydroponic Pea Media Preparation
- LSOP18 - Hydroponic Pea Sowing Seeds
- LSOP19 - In vitro screening of primary transformants (after floral dipping)
- LSOP20 - In vitro single node split plate assay Arabidopsis
- LSOP21 - Media Preparation for Adventitious Rooting (0.5% Sucrose)
- LSOP22 - Parasitic weed seed germination assay
- LSOP23 - Pea Grafting Procedure
- LSOP24 - Pea Seed Harvesting Protocol
- LSOP27 - Rules for Usage of Pea Seed
- LSOP29 - Soil screening of primary transformants (only for BASTA resistance)
- LSOP30 - Split-plate bud assay Pea
- LSOP32 - Time Lapse Protocol
- LSOP33 - Transformation of Arabidopsis by Floral Dipping
- LSOP34 - Tre6P Sugar Extractions
- LSOP39 - RNA extraction
- LSOP40 - UQ IBC Standard Operating Procedures for Quarantine Approved Premises
- LSOP41 - Using S4 Chemicals
- LSOP44 - Handling Disposal of PC2 Waste
- LSOP63 - Potting Soil for Pea
- LSOP64 - Collection and storage of Arabidopsis Seeds
- LSOP65 - Disposing of Plants
- LSOP66 - Washing Trays
- LSOP67 - Ordering for the Lab
- LSOP70 - Importing biological materials
- LSOP71 - Exporting biological materials
- LSOP72 - Submitting samples to AGRF
- LSOP73 - Harvesting of Plant Tissue
- LSOP75 - Handling Disposal of PC2 Sharps Waste
- LSOP78 - Working in Glasshouse
- LSOP98 - Field Visit
- LSOP99 - Preparation of auxin in lanolin for plant treatment
