In the interests of openness, we are pleased to share with you the Laboratory Standard Operating Procedures (LSOPs) for The University of Queensland Node. Please feel free to repurpose these to meet your own needs.
- LSOP01 - Adventitious Rooting Protocol
- LSOP02 - Arabidopsis Growing Procedure
- LSOP03 - Arabidopsis growth on Agar + seed sterilisation
- LSOP04 - Arabidopsis Protoplast Isolation
- LSOP05 - Arabidopsis Seed Surface Sterilisation
- LSOP06 - Phytohormone Extraction
- LSOP07 - Cleaning Procedure for Grinding Beads and Pestles
- LSOP08 - DNA extraction
- LSOP09 - Enzymatic Sugar Measurements
- LSOP11 - Exudate Collection from hydroponic Arabidopsis Roots
- LSOP12 - Fluorescamine Method to Calculate Total Amino Acids
- LSOP15 - How to make GR24 treatment solutions for direct pea bud applications
- LSOP16 - Hydroponic Arabidopsis
- LSOP17 - Hydroponic Pea Media Preparation
- LSOP18 - Hydroponic Pea Sowing Seeds
- LSOP20 - In vitro single node split plate assay Arabidopsis
- LSOP21 - Media Preparation for Adventitious Rooting (0.5% Sucrose)
- LSOP22 - Parasitic weed seed germination assay
- LSOP23 - Pea Grafting Procedure
- LSOP24 - Pea Seed Harvesting Protocol
- LSOP25 - Preparation of Thermo-Competent Agrobacterium
- LSOP26 - Protoplast Transfection
- LSOP27 - Rules for Usage of Pea Seed
- LSOP29 - Soil screening of primary transformants (only for BASTA resistance)
- LSOP30 - Split-plate bud assay Pea
- LSOP32 - Time Lapse Protocol
- LSOP33 - Transformation of Arabidopsis by Floral Dipping
- LSOP34 - Tre6P Sugar Extractions
- LSOP35 - Using the Vacuum Concentrator
- LSOP37 - Washing Up Procedure
- LSOP38 - Western Blot Protocol
- LSOP39 - RNA extraction
- LSOP40 - UQ IBC Standard Operating Procedures for Quarantine Approved Premises
- LSOP42 - Storing Items in Fridge and Freezers
- LSOP44 - Handling Disposal of PC2 Waste
- LSOP45 - Centrifuge Use
- LSOP46 - Using the microwave
- LSOP47 - Gel electrophoresis and imaging
- LSOP49 - Using the plate reader
- LSOP50 - Using the grinder
- LSOP51 - Refilling and using liquid nitrogen
- LSOP54 - Using Balances
- LSOP57 - Hotplates
- LSOP58 - Step Ladders
- LSOP59 - Scalpel Blade Use
- LSOP60 - Using Growth Cabinets
- LSOP62 - Freezer Operation (-20 and -80)
- LSOP65 - Disposing of Plants
- LSOP66 - Washing Trays
- LSOP68 - Pipette Usage
- LSOP78 - Working in Glasshouse
- LSOP83 - Autoclave
- LSOP94 - Waterbath Operation
- LSOP95 - Working in Cold Room
- LSOP98 - Field Visit
